HMGA2 Rabbit Polyclonal Antibody

CAT#: TA308757

Rabbit polyclonal antibody to HMGA2 (high mobility group AT-hook 2)

Product Images

Sample (30 ug of whole cell lysate). A: HepG2. B: HCT116. 15% SDS PAGE. TA308757 diluted at 1:10000.

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  Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using HMGA2 (TA308757) antibody at 1:500 dilution.

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  Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using HMGA2 (TA308757) antibody at 1:500 dilution.

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  Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using HMGA2 (TA308757) antibody at 1:500 dilution.

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  Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HMGA2 (TA308757) antibody at 1:500 dilution.

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  Immunoprecipitation of HMGA2 protein from HepG2 nuclear extracts using 5 ug of HMGA2 antibody (TA308757). Western blot analysis was performed using HMGA2 antibody (TA308757). An anti-rabbit IgG was used as a secondary reagent.

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Specifications

Product Data

• Applications: IF, IHC, IP, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:5000-1:20000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 109 of HMGA2 (Uniprot ID#P52926)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 12 kDa
• Gene Name: high mobility group AT-hook 2
• Database Link:
  NP_003475
  Entrez Gene 8091 Human
  UniProt ID P52926
• Background: This gene encodes a protein that belongs to the non-histone chromosomal high mobility group (HMG) protein family. HMG proteins function as architectural factors and are essential components of the enhancesome. This protein contains structural DNA-binding domains and may act as a transcriptional regulating factor. Identification of the deletion, amplification, and rearrangement of this gene that are associated with myxoid liposarcoma suggests a role in adipogenesis and mesenchymal differentiation. A gene knock out study of the mouse counterpart demonstrated that this gene is involved in diet-induced obesity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]
• Synonyms: BABL; HMGI-C; HMGIC; LIPO; STQTL9
• Note: Seq homology of immunogen across species: Pig (88%)

Reference Data

• Protein Families: Druggable Genome