UBE2L3 Rabbit Polyclonal Antibody

CAT#: TA308758

Rabbit Polyclonal antibody to UBE2L3 (ubiquitin-conjugating enzyme E2L 3)

Product Images

UBE2L3 antibody detects UBE2L3 protein by Western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. 15 % SDS-PAGE. UBE2L3 antibody (TA308758) dilution: 1:1000

• 

  Sample (30 ug of whole cell lysate). A: Hela. B: Hep G2. 12% SDS PAGE. TA308758 diluted at 1:1000.

• 

  Immunohistochemical analysis of paraffin-embedded A549 xenograft, using UBE2L3 (TA308758) antibody at 1:100 dilution.

• 

  Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using UBE2L3 (TA308758) antibody at 1:200 dilution.

• 

  Immunoprecipitation of UBE2L3 protein from HeLa whole cell extracts using 5 ug of UBE2L3 antibody (TA308758). Western blot analysis was performed using UBE2L3 antibody (TA308758). An anti-rabbit IgG was used as a secondary reagent.

Show all 5 images

Specifications

Product Data

• Applications: IF, IHC, IP, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a region within amino acids 92 and 154 of UBE2L3 (Uniprot ID#P68036)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 18 kDa
• Gene Name: ubiquitin conjugating enzyme E2 L3
• Database Link:
  NP_937800
  Entrez Gene 7332 Human
  UniProt ID P68036
• Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]
• Synonyms: E2-F1; L-UBC; UBCH7; UbcM4; ubiquitin-conjugating enzyme E2L 3; ubiquitin-conjugating enzyme UBCH7; ubiquitin-protein ligase; ubiquitin carrier protein
• Note: Seq homology of immunogen across species: Xenopus laevis (100%), Xenopus Tropicalis (100%), Pig (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%)

Reference Data

• Protein Pathways: Parkinson's disease, Ubiquitin mediated proteolysis