OGT Rabbit Polyclonal Antibody

CAT#: TA308773

Rabbit Polyclonal antibody to O-GlcNAc transferase (O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase))

Product Images

Sample (50 ug of whole cell lysate). A: Mouse brain. 5% SDS PAGE. TA308773 diluted at 1:1000.

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  Sample (30 ug of whole cell lysate). A: Hela. 7.5% SDS PAGE. TA308773 diluted at 1:1000

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  O-GlcNAc transferase antibody [N1N2], N-term detects O-GlcNAc transferase protein at cytosol on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. O-GlcNAc transferase antibody [N1N2], N-term (TA308773) dilution:

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  O-GlcNAc transferase antibody [N1N2], N-term detects OGT protein at cytosol on U373 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U373 xenograft. O-GlcNAc transferase antibody [N1N2], N-term (TA308773) dilution: 1:500.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 280 of O-GlcNAc transferase (Uniprot ID#O15294)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 116 kDa
• Gene Name: O-linked N-acetylglucosamine (GlcNAc) transferase
• Database Link:
  NP_858059
  Entrez Gene 26295 RatEntrez Gene 108155 MouseEntrez Gene 8473 Human
  UniProt ID O15294
• Background: O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]
• Synonyms: HRNT1; O-GLCNAC
• Note: Seq homology of immunogen across species: Rat (100%), Xenopus laevis (100%), Xenopus Tropicalis (100%), Pig (100%), Bovine (100%)

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, O-Glycan biosynthesis