Villin (VIL1) Rabbit Polyclonal Antibody

CAT#: TA308804

Rabbit Polyclonal antibody to Villin (villin 1)

Product Images

Villin antibody detects VIL1 protein by Western blot analysis. A. 50 ug mouse kidney lysate/extract. 7.5 % SDS-PAGE. Villin antibody (TA308804) dilution: 1:1000

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  Sample (30 ug of whole cell lysate). A: Hep G2. 7.5% SDS PAGE. TA308804 diluted at 1:5000

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  Villin antibody detects VIL1 protein by Western blot analysis. A. 50 ug rat kidney lysate/extract. 7.5 % SDS-PAGE. Villin antibody (TA308804) dilution: 1:2000

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  Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Villin (TA308804) antibody at 1:500 dilution.

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  Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Villin (TA308804) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2000.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:1000-1:10000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 7 and 246 of Villin
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 93 kDa
• Gene Name: villin 1
• Database Link:
  NP_009058
  Entrez Gene 7429 Human
  UniProt ID P09327
• Background: This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. [provided by RefSeq]
• Synonyms: D2S1471; VIL