CAD Rabbit Polyclonal Antibody

CAT#: TA308818

Rabbit polyclonal antibody to CAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase)

Product Images

CAD antibody [C2C3], C-term detects CAD protein by Western blot analysis. A. 30 ug C2C12 whole cell lysate/extract. 5 % SDS-PAGE. CAD antibody [C2C3], C-term (TA308818) dilution: 1:1000

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  Sample (30ug whole cell lysate). A:293T whole cell lysate. 5% SDS PAGE. TA308818 diluted at 1:1500

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  Immunohistochemical analysis of Salivary gland cancer, using CAD (TA308818) antibody at 1:100 dilution.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1625 and 1930 of CAD (Uniprot ID#P27708)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 243 kDa
• Gene Name: carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
• Database Link:
  NP_004332
  Entrez Gene 790 Human
  UniProt ID P27708
• Background: The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. [provided by RefSeq]
• Synonyms: and dihydroorotase; aspartate transcarbamylase; CAD trifunctional protein; carbamoyl-phosphate synthetase 2; carbamoylphosphate synthetase 2; dihydroorotase; multifunctional protein CAD

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Metabolic pathways, Pyrimidine metabolism