beta Actin (ACTB) Rabbit Polyclonal Antibody

CAT#: TA308840

Rabbit Polyclonal antibody to beta Actin (actin, beta)

Product Images

Sample (30 ug of whole cell lysate). A: A431. B: H1299. 10% SDS PAGE. TA308840 diluted at 1:1000.

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  Immunohistochemical analysis of paraffin-embedded Prostate, using beta Actin (TA308840) antibody (10 ug/ml).

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  Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using beta Actin (TA308840) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2000.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide contain a sequence corresponding to a region within amino acids 309 and 358 of beta Actin
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 42 kDa
• Gene Name: actin, beta
• Database Link:
  NP_001092
  Entrez Gene 11461 MouseEntrez Gene 81822 RatEntrez Gene 403580 DogEntrez Gene 60 Human
  UniProt ID P60709
• Background: This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]
• Synonyms: BRWS1; PS1TP5BP1
• Note: Seq homology of immunogen across species: Mouse (100%), Rat (100%), Dog (100%), Rabbit (100%), Chicken (100%), Sheep (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%), Guinea pig (100%)

Reference Data

• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, Tight junction, Vibrio cholerae infection, Viral myocarditis