Alkaline Phosphatase (ALPL) Rabbit Polyclonal Antibody

CAT#: TA308847

Rabbit polyclonal antibody to alkaline phosphatase (liver/bone/kidney) (alkaline phosphatase, liver/bone/kidney)

Product Images

Sample (30 ug of whole cell lysate). A: Hela. 7.5% SDS PAGE. TA308847 diluted at 1:1000.

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  Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using alkaline phosphatase (liver/bone/kidney) (TA308847) antibody at 1:100 dilution.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment contain a sequence corresponding to a region within amino acids 1 and 359 of Alkaline Phosphatase (Tissue Non-Specific) (Uniprot ID#P05186)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 57 kDa
• Gene Name: alkaline phosphatase, liver/bone/kidney
• Database Link:
  NP_001120973
  Entrez Gene 11647 MouseEntrez Gene 25586 RatEntrez Gene 403548 DogEntrez Gene 249 Human
  UniProt ID P05186
• Background: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq]
• Synonyms: AP-TNAP; APTNAP; HOPS; TNAP; TNSALP
• Note: Seq homology of immunogen across species: Mouse (91%), Cat (90%), Dog (89%), Rat (91%), Bovine (89%)

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Folate biosynthesis, Metabolic pathways