COX4 (COX4I1) Rabbit Polyclonal Antibody

CAT#: TA308869

Rabbit Polyclonal antibody to COX4 (cytochrome c oxidase subunit IV isoform 1)

Product Images

Sample (30 ug of whole cell lysate). A:A431. B:MOLT4. 12% SDS PAGE. TA308869 diluted at 1:2000

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  Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using COX4 (TA308869) antibody at 1:100 dilution.

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  Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using COX4 (TA308869) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2000.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 169 of COX4 (Uniprot ID#P13073)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 20 kDa
• Gene Name: cytochrome c oxidase subunit 4I1
• Database Link:
  NP_001852
  Entrez Gene 1327 Human
  UniProt ID P13073
• Background: Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq]
• Synonyms: COX4; COX4-1; COXIV; COX IV-1; COXIV-1
• Note: Seq homology of immunogen across species: Chimpanzee (100%)

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Alzheimer's disease, Cardiac muscle contraction, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease