GAPDH Rabbit Polyclonal Antibody

CAT#: TA308884

Rabbit Polyclonal antibody to GAPDH (glyceraldehyde-3-phosphate dehydrogenase)

Product Images

GAPDH antibody detects GAPDH protein by Western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug NIH-3T3 whole cell lysate/extract. C. 30 ug mouse brain lysate/extract. D. 30 ug PC-12 whole cell lysate/extract. E. 30 ug rat brain lysate/ex

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  GAPDH antibody detects GAPDH protein by Western blot analysis. A. 0.1 ug 293T whole cell lysate/extract. B. 1 ug 293T whole cell lysate/extract. C. 10 ug 293T whole cell lysate/extract. 12 % SDS-PAGE. GAPDH antibody (TA308884) dilution: 1:10000

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  GAPDH antibody detects GAPDH protein by western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug A431 whole cell lysate/extract. C. 30 ug HeLa whole cell lysate/extract |. D. 30 ug HepG2 whole cell lysate/extract. 10 % SDS-PAGE. GAPDH anti

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  GAPDH antibody detects GAPDH protein by western blot analysis. A. 20 ug 293T whole cell lysate/extract. B. 10 ug 293T whole cell lysate/extract. C. 5 ug 293T whole cell lysate/extract. D. 1 ug 293T whole cell lysate/extract. 10 % SDS-PAGE. GAPDH antibody

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  GAPDH antibody detects GAPDH protein by western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract. E. 30 ug BCL-1 whole

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  GAPDH antibody detects GAPDH protein by western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. B. 30 ug Rat2 whole cell lysate/extract. 10 % SDS-PAGE. GAPDH antibody (TA308884) dilution: 1:10000

• Figure from citation: Western blot analysis of GAPDH protein expression by using anti-GAPDH antibody in NCI-N87 cells. Dilution: 1:10000 View Citation

• Immunohistochemical analysis of Salivary gland cancer, using GAPDH (TA308884) antibody at 1:500 dilution.

• Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GAPDH (TA308884) antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (red) at 1:2000.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:5000-1:20000
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 335 of GAPDH (Uniprot ID#P04406)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 36 kDa
• Gene Name: glyceraldehyde-3-phosphate dehydrogenase
• Database Link:
  NP_002037
  Entrez Gene 14433 MouseEntrez Gene 403755 DogEntrez Gene 574353 MonkeyEntrez Gene 2597 Human
  UniProt ID P04406
• Background: The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]
• Synonyms: G3PD; GAPD; HEL-S-162eP
• Note: Seq homology of immunogen across species: Cat (96%), Chicken (92%), Dog (95%), Monkey (99%), Pig (95%), Rabbit (94%), Xenopus laevis (85%), Zebrafish (86%), Bovine (94%)

Reference Data

• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: Alzheimer's disease, Glycolysis / Gluconeogenesis, Metabolic pathways