H3.3B (H3F3B) Rabbit Polyclonal Antibody

CAT#: TA308895

Rabbit polyclonal anti-H3F3B(Histone H3) antibody, Loading control

Product Images

Sample (50 ug of whole cell lysate). A: mouse brain. 15% SDS PAGE. TA308895 diluted at 1:1000.

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  Histone H3.3B antibody detects H3F3B protein by Western blot analysis. A. 50 ug rat brain lysate/extract. 15 % SDS-PAGE. Histone H3.3B antibody (TA308895) dilution: 1:1000

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  Sample (30 ug of whole cell lysate). A: Hela. 15% SDS PAGE. TA308895 diluted at 1:1000.

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  Histone H3.3B antibody detects Histone H3.3B protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Histone H3.3B antibody (TA308895) dilution: 1:500.

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  Histone H3.3B antibody detects Histone H3.3B protein at nucleus on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. Histone H3.3B antibody (TA308895) dilution: 1:500.

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  Immunohistochemical analysis of paraffin-embedded HSC3 xenograft, using Histone H3.3B (TA308895) antibody at 1:500 dilution.

• Histone H3.3B antibody detects Histone H3.3B protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3.3B protein stained by Histone H3.3B antibody (TA308895) diluted at 1:50

• Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Histone H3.3B (TA308895) antibody at 1:200 dilution.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500 - 1:3000, IHC 1:100 - 1:1000, IF 1:100 - 1:1000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 86 of Histone H3.3B
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 15 kDa
• Gene Name: H3 histone, family 3B (H3.3B)
• Database Link:
  NP_005315
  Entrez Gene 15081 MouseEntrez Gene 117056 RatEntrez Gene 3021 Human
• Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is poyadenylated, unlike most histone genes. The protein encoded is a member of the histone H3 family. [provided by RefSeq]
• Synonyms: H3.3B
• Note: Seq homology of immunogen across species: Xenopus laevis (100%), Xenopus Tropicalis (100%), Chicken (100%), Bovine (100%)

Reference Data

• Protein Pathways: Systemic lupus erythematosus