HSP70-1A (HSPA1A) Rabbit Polyclonal Antibody

CAT#: TA308905

Rabbit polyclonal anti-HSPA1A(HSP70) antibody, Loading control

Product Images

Sample (whole cell lysate). A: 293T 20ug. B: 293T 10ug. C: 293T 5ug. D: 293T 1ug. 7.5% SDS PAGE. TA308905 diluted at 1:10000.

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  Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. TA308905 diluted at 1:10000.

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  Sample (30 ug of whole cell lysate). A: Jurkat. B: Raji. C: 293T. D: A431. E: HeLa. F: HepG2. G: H1299. H: HCT116. I: MCF-7. J: NT2D1. K: PC-3. L: U87-MG. 7.5% SDS PAGE. TA308905 diluted at 1:10000.

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  Sample (30 ug of whole cell lysate). A: 293T. B: NIH-3T3. C: Mouse brain. D: PC-12. E: Rat brain. 7.5% SDS PAGE. TA308905 diluted at 1:10000.

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  HSP70 1A antibody detects HSPA1A protein at cytosol on H1299 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded H1299 xenograft. HSP70 1A antibody (TA308905) dilution: 1:500.

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  Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using HSP70 1A (TA308905) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2000.

• HSP70 1A antibody immunoprecipitates HSP70 1A protein in IP experiments. IP Sample: 1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2.5 ug of preimmune rabbit IgG C. Immunoprecipitation of HSP70 1A protein by

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Specifications

Product Data

• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB 1:5000 - 1:20000, IHC 1:100 - 1:1000, ICC/IF 1:100 - 1:1000
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 308 and 569 of HSP70 1A
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 70 kDa
• Gene Name: heat shock protein family A (Hsp70) member 1A
• Database Link:
  NP_005336
  Entrez Gene 193740 MouseEntrez Gene 3303 Human
  UniProt ID P0DMV8
• Background: This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq]
• Synonyms: HEL-S-103; HSP70-1; HSP70-1A; HSP70.1; HSP70I; HSP72; HSPA1
• Note: Seq homology of immunogen across species: Sheep (100%), Bovine (100%)

Reference Data

• Protein Pathways: Antigen processing and presentation, Endocytosis, MAPK signaling pathway, Prion diseases, Spliceosome