LIM kinase 2 (LIMK2) Rabbit Polyclonal Antibody

CAT#: TA308918

Rabbit polyclonal antibody to LIM kinase 2 (LIM domain kinase 2)

Product Images

Sample (30ug whole cell lysate). A:293T. 7.5% SDS PAGE. TA308918 diluted at 1:1000

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  Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using LIM kinase 2 (TA308918) antibody at 1:500 dilution.

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  Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using LIM kinase 2 (TA308918) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2000.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment contain a sequence corresponding to a region within amino acids 294 and 638 of LIM kinase 2
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 78 kDa
• Gene Name: LIM domain kinase 2
• Database Link:
  NP_001026971
  Entrez Gene 3985 Human
  UniProt ID P53671
• Background: There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
• Synonyms: LIMK-2

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton