MMP2 Rabbit Polyclonal Antibody

CAT#: TA308925

Rabbit Polyclonal antibody to MMP2 (matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase))

Product Images

Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. TA308925 diluted at 1:1000.

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  MMP2 antibody detects MMP2 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP2 antibody (TA308925) diluted by 1:3000.

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  Immunohistochemical analysis of paraffin-embedded U251 xenograft, using MMP2 (TA308925) antibody at 1:500 dilution.

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  Immunofluorescence analysis of methanol-fixed HeLa, using MMP2 (TA308925) antibody at 1:200 dilution.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 193 and 575 of MMP2 (Uniprot ID#P08253)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 74 kDa
• Gene Name: matrix metallopeptidase 2
• Database Link:
  NP_001121363
  Entrez Gene 17390 MouseEntrez Gene 4313 Human
  UniProt ID P08253
• Background: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
• Synonyms: CLG4; CLG4A; MMP-2; MMP-II; MONA; TBE-1
• Note: Seq homology of immunogen across species: Xenopus laevis (81%), Bovine (94%)

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Bladder cancer, GnRH signaling pathway, Leukocyte transendothelial migration, Pathways in cancer