BNP (NPPB) Rabbit Polyclonal Antibody

CAT#: TA308929

Rabbit polyclonal antibody to BNP (natriuretic peptide precursor B)

Product Images

BNP antibody detects BNP protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with BNP antibody (TA308929) diluted by 1:1000.

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  BNP antibody [N1C3] detects BNP protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: BNP protein stained by BNP antibody [N1C3] (TA308929) diluted at 1:500. Blue: Hoechst 33342 staining.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 134 of BNP (Uniprot ID#P16860)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 15 kDa
• Gene Name: natriuretic peptide B
• Database Link:
  NP_002512
  Entrez Gene 4879 Human
  UniProt ID P16860
• Background: This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. Mutations in this gene have been associated with postmenopausal osteoporosis. [provided by RefSeq]
• Synonyms: BNP

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Stem cell - Pluripotency