PKC alpha (PRKCA) Rabbit Polyclonal Antibody

CAT#: TA308938

Rabbit Polyclonal antibody to PKC alpha (protein kinase C, alpha)

Product Images

Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. TA308938 diluted at 1:1000.

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  Sample (30 ug of whole cell lysate). A: H1299. 7.5% SDS PAGE. TA308938 diluted at 1:1000.

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  Immunohistochemical analysis of paraffin-embedded Colon ca, using PKC alpha (TA308938) antibody at 1:500 dilution.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 250 of PKC alpha (Uniprot ID#P17252)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 77 kDa
• Gene Name: protein kinase C alpha
• Database Link:
  NP_002728
  Entrez Gene 18750 MouseEntrez Gene 5578 Human
  UniProt ID P17252
• Background: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq]
• Synonyms: AAG6; PKC-alpha; PKCA; PRKACA
• Note: Seq homology of immunogen across species: Rhesus Monkey (100%)

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
• Protein Pathways: Calcium signaling pathway, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, Leukocyte transendothelial migration, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Natural killer cell mediated cytotoxicity, Non-small cell lung cancer, Pathogenic Escherichia coli infection, Pathways in cancer, Phosphatidylinositol signaling system, Tight junction, Vascular smooth muscle contraction, VEGF signaling pathway, Vibrio cholerae infection, Wnt signaling pathway