ROCK1 Rabbit Polyclonal Antibody

CAT#: TA308951

Rabbit polyclonal antibody to ROCK1 (Rho-associated, coiled-coil containing protein kinase 1)

Product Images

ROCK1 antibody [N1N2], N-term detects ROCK1 protein by western blot analysis. A. 30 ug NIH-3T3 whole cell extract. B. 30 ug C2Cl2 whole cell extract. 5 % SDS-PAGE. ROCK1 antibody [N1N2], N-term (TA308951) dilution: 1:1000

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  ROCK1 antibody [N1N2], N-term detects ROCK1 protein by Western blot analysis. A. 50 ug rat brain lysate/extract. 5 % SDS-PAGE. ROCK1 antibody [N1N2], N-term (TA308951) dilution: 1:500

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  Sample (30 ug of whole cell lysate). A: A431. B: H1299. C: Molt-4. 5% SDS PAGE. TA308951 diluted at 1:1000.

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  Immunohistochemical analysis of paraffin-embedded DLD1 xenograft, using ROCK1 (TA308951) antibody at 1:500 dilution.

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  Immunofluorescence analysis of methanol-fixed HeLa, using ROCK1 (TA308951) antibody at 1:200 dilution.

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  ROCK1 antibody immunoprecipitates ROCK1 protein in IP experiments. IP Sample: A431 whole cell lysate/extract A : Control with 3 ug of pre-immune rabbit IgG B : Immunoprecipitation of ROCK1 by 3 ug of ROCK1 antibody (TA308951) 5% SDS-PAGE The immunoprecipi

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Specifications

Product Data

• Applications: IF, IHC, IP, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 107 and 371 of ROCK1
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 158 kDa
• Gene Name: Rho associated coiled-coil containing protein kinase 1
• Database Link:
  NP_005397
  Entrez Gene 6093 Human
  UniProt ID Q13464
• Background: This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. [provided by RefSeq]
• Synonyms: P160ROCK; ROCK-I

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Axon guidance, Chemokine signaling pathway, Focal adhesion, Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, TGF-beta signaling pathway, Vascular smooth muscle contraction, Wnt signaling pathway