UNC13B Rabbit Polyclonal Antibody

CAT#: TA308990

Rabbit Polyclonal antibody to UNC13B (unc-13 homolog B (C. elegans))

Product Images

Sample (30 ug of whole cell lysate). A: 293T. 5% SDS PAGE. TA308990 diluted at 1:500.

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  Immunohistochemical analysis of paraffin-embedded A549 xenograft, using UNC13B (TA308990) antibody at 1:500 dilution.

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  Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using UNC13B (TA308990) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2000.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 680 and 957 of UNC13B (Uniprot ID#O14795)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 181 kDa
• Gene Name: unc-13 homolog B (C. elegans)
• Database Link:
  NP_006368
  Entrez Gene 22249 MouseEntrez Gene 64830 RatEntrez Gene 10497 Human
  UniProt ID O14795
• Background: This gene is expressed in the kidney cortical epithelial cells and is upregulated by hyperglycemia. The encoded protein shares a high level of similarity to the rat homolog, and contains 3 C2 domains and a diacylglycerol-binding C1 domain. Hyperglycemia increases the levels of diacylglycerol, which has been shown to induce apoptosis in cells transfected with this gene and thus contribute to the renal cell complications of hyperglycemia. Studies in other species also indicate a role for this protein in the priming step of synaptic vesicle exocytosis. [provided by RefSeq]
• Synonyms: MUNC13; UNC13; Unc13h2
• Note: Seq homology of immunogen across species: Mouse (99%), Rat (98%), Bovine (99%)

Reference Data

• Protein Families: Druggable Genome