Mapk1 Rabbit Polyclonal Antibody

CAT#: TA309121

Rabbit Anti-ERK/MAPK (Thr202/Tyr204) Antibody (Phospho-Specific)

Product Images

Western blot of human T47D cell lysates showing specific immunolabeling of ~42-44k ERK/MAPK protein phosphorylated at Thr202 and Tyr204 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that it was incubated in λ-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr202/Tyr204 ERK/MAPK. The immunolabeling is completely eliminated by treatment with λ-Ptase.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:1000, IHC: 1:500
• Reactivities: Human, Rat
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr202/Tyr204 conjugated to KLH
• Formulation: 100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 42/44 kDa
• Gene Name: mitogen activated protein kinase 1
• Database Link:
  NP_446294
  Entrez Gene 5594 HumanEntrez Gene 116590 Rat
  UniProt ID P63086
• Synonyms: ERK; ERK-2; ERK2; ERT1; MAPK2; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; PRKM1; PRKM2