Vim Chicken Polyclonal Antibody

CAT#: TA309274

Chicken Anti-Vimentin Antibody

Product Images

Western blot of NIH 3T3 cells showing specific immunolabeling of the ~50k Vimentin protein.

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  Mixed neuron/glial cultures stained with anti-Vimentin (green) and rabbit anti-GFAP antibody (red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and vimentin, the two proteins assemble to produce heteropolymer filaments.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1:1,000, IF: 1:500
• Reactivities: Rat
• Host: Chicken
• Isotype: IgY
• Clonality: Polyclonal
• Immunogen: Recombinant human vimentin purified from E. coli
• Formulation: total IgY fraction in PBS + 10 mM NaN3.
• Purification: Total IgY fraction
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50 kDa
• Gene Name: vimentin
• Database Link:
  NP_112402
  Entrez Gene 81818 Rat
  UniProt ID P31000
• Background: This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.
• Synonyms: FLJ36605; vimentin