Ubiquitin (UBB) Rabbit Polyclonal Antibody

CAT#: TA309498

Rabbit Polyclonal anti-UBB Antibody

Product Images

Western blot analysis of ubiquitinated proteins in HEK93 cells, using a dilution of anti-UBB.

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  Immunohistochemistry analysis using Rabbit Anti-Ubiquitin Polyclonal Antibody. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody at 1:100000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT.

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  Ubiquitin visualized using anti-UBB, tested on Bouin's fixed paraffin-embedded backskin sections of transgenic mice.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB, IP
• Reactivities: Human, Monkey, Mouse, Rat, Hamster, Rabbit, Guinea Porcine, Bovine, Porcine, Dog, Sheep, Chicken, Xenopus, Drosophila
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Native bovine Ubiquitin, conjugated to KLH
• Formulation: PBS pH7.4, 50% glycerol
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: ubiquitin B
• Database Link:
  NP_061828
  Entrez Gene 22187 MouseEntrez Gene 192255 RatEntrez Gene 479513 DogEntrez Gene 696110 MonkeyEntrez Gene 7314 Human
  UniProt ID P0CG47
• Background: This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome. [provided by RefSeq]
• Synonyms: HEL-S-50

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Parkinson's disease