Aquaporin 2 (AQP2) Rabbit Polyclonal Antibody

CAT#: TA309574

Rabbit Polyclonal Aquaporin-2 Antibody

Product Images

Western Blot: Aquaporin-2 Antibody TA309574 - Detection of Aquaporin 2 in rat kidney extracts. Cortex (CX), Outer Medulla (OM), Inner Medulla Base (IMB), Inner Medulla Papilla (IMP).

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  Immunocytochemistry/Immunofluorescence: Aquaporin-2 Antibody TA309574 - Staining of mpkCCD cell line

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  Immunocytochemistry/Immunofluorescence: Aquaporin-2 Antibody TA309574 - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Aquaporin-2 Antibody TA309574 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

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  Immunocytochemistry/Immunofluorescence: Aquaporin-2 Antibody TA309574 - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Aquaporin-2 Antibody TA309574 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Immunohistochemistry-Frozen, Immunohistochemistry: 1:100-1:200, Immunohistochemistry-Paraffin: 1:100-1:200, Western Blot: 1 - 2 ug/ml, Immunocytochemistry/ Immunofluorescence: 1 ug/ml
• Reactivities: Bovine, Human, Mouse, Porcine, Rat, Sheep
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide made to a C-terminus portion of the rat protein (within residues 200-300). [Swiss-Prot# P34080]
• Formulation: PBS and 30% Glycerol and 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Peptide affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: aquaporin 2
• Database Link:
  NP_000477
  Entrez Gene 11827 MouseEntrez Gene 25386 RatEntrez Gene 359 Human
  UniProt ID P41181
• Synonyms: AQP-CD; WCH-CD

Reference Data

• Protein Families: Druggable Genome, Transmembrane