BRCA1 Mouse Monoclonal Antibody [Clone ID: MU]

CAT#: TA309590

Mouse Monoclonal BRCA1 Antibody (MU)

Product Images

Flow Cytometry: BRCA1 Antibody (MU) TA309590 - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using BRCA1 antibody (dark blue). Isotype control shown in orange. Antibody at 1 ug/1 x 10^6 cells was used.

• 

  Flow Cytometry: BRCA1 Antibody (MU) TA309590 - An intracellular stain was performed on MCF7 cells with BRCA1 Antibody [MU] [TA309590C] (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.

• 

  Western Blot: BRCA1 Antibody (MU) TA309590 - Detection of BRCA1 using TA309590.

• 

  Western Blot: BRCA1 Antibody (MU) TA309590 - Detection of BRCA1 in MCF-7 whole cell lysate using TA309590. 2 minute ECL exposure.

Specifications

Product Data

• Clone Name: MU
• Applications: WB
• Recommended Dilution: Western Blot: 2 - 4 ug/mL, Immunoprecipitation: 1:10 - 1:500, Flow Cytometry: 1 ug per million cells
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b, kappa
• Clonality: Monoclonal
• Immunogen: Human BRCA1 (residues 1314-1864).
• Formulation: Tris-glycine, 150mM NaCl pH 7.5 and 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: BRCA1, DNA repair associated
• Database Link:
  NP_009225
  Entrez Gene 672 Human
  UniProt ID P38398
• Synonyms: BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4; PPP1R53; PSCP; RNF53

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Ubiquitin mediated proteolysis