KDM4C Rabbit Polyclonal Antibody

CAT#: TA309711

Rabbit Polyclonal JMJD2C Antibody

Product Images

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody TA309711 - Staining of JMJD2C in paraffin embedded mouse pancreas using DAB with hematoxylin counterstain.

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  Simple Western: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody TA309711 - Simple Western lane view shows a specific band for JMJD2C in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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  Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody TA309711 - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody TA309711 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

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  Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody TA309711 - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody TA309711 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

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  Western Blot: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody TA309711 - Analysis of JMJD2C in HeLa nuclear extracts.

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry: 1:100, Immunocytochemistry/ Immunofluorescence: 1:100, Immunohistochemistry-Paraffin: 1:100, Simple Western: 1:12.5, Western Blot: 1:1000, Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation, Immunoprecipitation
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A genomic peptide made to an internal region of the human JMJD2C protein (within residues 450-600). [Swiss-Prot Q9H3R0]
• Formulation: PBS, 50% glycerol and 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: lysine demethylase 4C
• Database Link:
  NP_001140166
  Entrez Gene 76804 MouseEntrez Gene 23081 Human
  UniProt ID Q9H3R0
• Synonyms: bA146B14.1; GASC1; JHDM3C; JMJD2C; TDRD14C

Reference Data

• Protein Families: Druggable Genome, Transcription Factors