OPA1 Rabbit Polyclonal Antibody

CAT#: TA309781

Rabbit Polyclonal OPA1 Antibody

Product Images

Immunohistochemistry: OPA1 Antibody TA309781 - Staining in prostatic smooth muscle and glandular epithelium. Human Prostate 40X magnification.

• 

  Simple Western: OPA1 Antibody TA309781 - Lane view shows a specific band for OPA1 in 0.5 mg/ml of MEF lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• 

  Western Blot: OPA1 Antibody TA309781 - Detection of OPA1 protein in post-nuclear extracts of mouse embryonic fibroblasts.

• 

  Western Blot: OPA1 Antibody TA309781 - Total protein from Human HeLa cells, Mouse MEF cells and Rat Brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-OPA1 in 1% blocking buffer. Precision Plus Protein All Blue molecular weight markers (BioRad) were detected with 1 ug/ml Anti-Blue Marker antibody (NBP2-33376). The protein ladder was detected with an anti-mouse HRP secondary antibody and OPA1 with an anti-rabbit HRP secondary antibody using chemiluminescence.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Immunocytochemistry/ Immunofluorescence, Immunoblotting, Western Blot: 2 ug/ml, Immunohistochemistry-Paraffin: 2.5 ug/ml, Simple Western: 20 ug/ml, Immunohistochemistry: 2.5 ug/ml
• Reactivities: Chicken, Human, Mouse, Primate, Rat, Zebrafish
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide made to an internal region within residues 500-600 of the human protein. [Swiss-Prot# O60313]
• Formulation: Tris-glycine, 150mM NaCl and 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: OPA1, mitochondrial dynamin like GTPase
• Database Link:
  NP_570850
  Entrez Gene 74143 MouseEntrez Gene 171116 RatEntrez Gene 4976 Human
  UniProt ID O60313
• Synonyms: BERHS; largeG; MGM1; MTDPS14; NPG; NTG