PKM2 (PKM) Rabbit Polyclonal Antibody

CAT#: TA309804

Rabbit Polyclonal PKM2 Antibody

Product Images

Immunohistochemistry: PKM2 Antibody - BSA Free TA309804 - Staining of PKM2 in mouse liver tissue.

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  Immunocytochemistry/Immunofluorescence: PKM2 Antibody - BSA Free TA309804 - Analysis of PKM2 in HeLa cells.

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  Simple Western: PKM2 Antibody - BSA Free TA309804 - Lane view shows a specific band for PKM2 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

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  Flow Cytometry: PKM2 Antibody - BSA Free TA309804 - An intracellular stain was performed on HeLa cells with PKM2 Antibody TA309804AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

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  Flow Cytometry: PKM2 Antibody - BSA Free TA309804 - An intracellular stain was performed on Neuro2a cells with PKM2 Antibody TA309804AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

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  Western Blot: PKM2 Antibody - BSA Free TA309804 - Analysis of PKM2 in MCF7 whole cell lysates.

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Specifications

Product Data

• Applications: WB
• Recommended Dilution: Flow Cytometry: 5 ug/ml, Knockdown Validated, Western Blot: 0.5 ug/ml, Immunohistochemistry: 1:100, Immunohistochemistry-Paraffin: 1:100, Simple Western: 1:12.5, Immunocytochemistry/ Immunofluorescence: 1:200, Immunoprecipitation, Immunoblotting
• Reactivities: Bovine, Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide made to the internal region of human PKM2 protein (within residues 350-450). [Swiss-Prot# P14618]
• Formulation: PBS, 30% glycerol and 0.1% Sodium Azide
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: pyruvate kinase, muscle
• Database Link:
  NP_872270
  Entrez Gene 18746 MouseEntrez Gene 25630 RatEntrez Gene 5315 Human
  UniProt ID P14618
• Synonyms: CTHBP; HEL-S-30; OIP3; PK3; PKM2; TCB; THBP1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Glycolysis / Gluconeogenesis, Metabolic pathways, Purine metabolism, Pyruvate metabolism, Type II diabetes mellitus