Perilipin-1 (PLIN1) Rabbit Polyclonal Antibody

CAT#: TA309806

Rabbit Polyclonal Perilipin Antibody

Product Images

Western Blot: Perilipin Antibody TA309806 - Total protein from Human breast (lane 1), Human adipose membrane fraction (lane 2) and Human adipose tissue (lane 3) were separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane, probed with 2 ug/mL anti-perilipin antibody followed by anti-rabbit HRP conjugated secondary antibody and detected with chemiluminescence.

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  Flow Cytometry: Perilipin Antibody TA309806 - An intracellular stain was performed on U2OS cells with Perilipin Antibody TA309806AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

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  Flow Cytometry: Perilipin Antibody TA309806 - An intracellular stain was performed on MCF7 cells with Perilipin Antibody TA309806AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

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  Immunohistochemistry-Paraffin: Perilipin Antibody TA309806 - IHC analysis of formalin fixed paraffin embedded section of fat tissue from mouse with Perilipin antibody at 1:200 dilution. The antibody generated an expected staining in the adipocytes towards periphery of the cells.

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  Immunohistochemistry-Paraffin: Perilipin Antibody TA309806 - IHC analysis of formalin fixed paraffin embedded section of liver tissue from mouse with Perilipin antibody at 1:200 dilution. The hepatocytes developed specific staining in the cytoplasm and around the nuclei of some cells, the immunostaining reflected a punctate appearance which is potentially the ER of the cells.

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  Immunohistochemistry-Paraffin: Perilipin Antibody TA309806 - IHC analysis of formalin fixed paraffin embedded section of liver tissue from mouse with Perilipin antibody at 1:200 dilution. The hepatocytes developed specific staining in the cytoplasm and around the nuclei of some cells, the immunostaining reflected a punctate appearance which is potentially the ER of the cells.

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Specifications

Product Data

• Applications: WB
• Recommended Dilution: Imaging Mass Cytometry, Western Blot: 2 ug/mL, Flow Cytometry: 5 - 10 ug/mL, Immunohistochemistry-Paraffin: 1:200, Immunohistochemistry: 1:200, Immunocytochemistry/ Immunofluorescence
• Reactivities: Bovine, Human, Mouse, Porcine, Rat, Sheep
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide made to a region between residues 450-522 (C-terminus) of the human perilipin protein. [Swiss-Prot# O60240]
• Formulation: Tris-glycine, 150mM NaCl and 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Peptide affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: perilipin 1
• Database Link:
  NP_001138783
  Entrez Gene 25629 RatEntrez Gene 103968 MouseEntrez Gene 5346 Human
  UniProt ID O60240
• Synonyms: FPLD4; PERI; PLIN

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: PPAR signaling pathway