PODXL Rabbit Polyclonal Antibody

CAT#: TA309811

Rabbit Polyclonal Podocalyxin Antibody

Product Images

Immunocytochemistry/Immunofluorescence: Podocalyxin Like Antibody TA309811 - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Podocalyxin Like Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

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  Western Blot: Podocalyxin Like Antibody TA309811 - Analysis of Podolyxin in 1. Human thymus and 2. Human lymph node.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western Blot: 2 ug/ml, Immunocytochemistry/ Immunofluorescence: 1:1000
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide within an internal region (residues 100-200) of the human Podocalyxin protein. [Swiss-Prot# O00592] This immunogen should be far enough away from any glycosylation site.
• Formulation: Tris-glycine, 150mM NaCl and 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: podocalyxin like
• Database Link:
  NP_005388
  Entrez Gene 5420 Human
  UniProt ID O00592
• Synonyms: Gp200; PC; PCLP; PCLP-1

Reference Data

• Protein Families: Transmembrane