EEF1G Rabbit Monoclonal Antibody [Clone ID: EPR7200]

CAT#: TA310440

Rabbit Monoclonal antibody against EEF1G

Product Images

Western blot - Anti-EEF1G antibody [EPR7200]; All lanes : Anti-EEF1G antibody [EPR7200] at 1/10000 dilution.Lane 1 : 293T cell lysate.Lane 2 : HL60 cell lysate.Lane 3 : C6 cell lysate.Lane 4 : RAW 264.7 cell lysate.Lane 5 : PC12 cell lysate.Lane 6 : NIH 3T3 cell lysate.Lane 7 : HepG2 cell lysate.Lane 8 : HeLa cell lysate.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution.Predicted band size : 50 kDa.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF1G antibody [EPR7200]; TA310440 at 1/250 dilution staining EEF1G in paraffin-embedded Human pancreatic adenocarcinoma tissue by Immunohistochemistry.

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  Immunocytochemistry/ Immunofluorescence - Anti-EEF1G antibody [EPR7200]; TA310440 at 1/100 dilution staining EEF1G in HeLa cells by Immunofluorescence.

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  Other-Anti-EEF1G antibody [EPR7200] (TA310440); Equilibrium disassociation constant (KD).

Specifications

Product Data

• Clone Name: EPR7200
• Applications: Assay, IF, IHC, WB
• Recommended Dilution: WB: 1:10000 - 1:50000; IP: 1:10 - 1:100; IHC-P: 1:250 - 1:500; FC: 1:10 - 1:100; ICC/IF: 1:100 - 1:250
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide corresponding to residues in human EEF1G was used as an immunogen.
• Formulation: pH: 7.20
  Preservative: 0.01% Sodium azide
  Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
• Purification: Tissue culture supernatant
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50 kDa
• Gene Name: eukaryotic translation elongation factor 1 gamma
• Database Link:
  NP_001395
  Entrez Gene 67160 MouseEntrez Gene 293725 RatEntrez Gene 1937 Human
  UniProt ID P26641
• Background: EEF1G is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases (1).
• Synonyms: EF1G; GIG35