GLI1 Rabbit Monoclonal Antibody [Clone ID: EPR4523]

CAT#: TA310536

Rabbit Monoclonal antibody against Gli-1 (GLI1)

Product Images

Western blot - Anti-Gli1 antibody [EPR4523]; All lanes : Anti-Gli1 antibody [EPR4523] at 1/1000 dilution.Lane 1 : RMS13 cell lysate.Lane 2 : A673 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP-conjugated goat anti-rabbit IgG polyclonal at 1/2000 dilution.Predicted band size : 118 kDa.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gli1 antibody [EPR4523]; Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling Gli1 with TA310536 at 1/250 dilution.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gli1 antibody [EPR4523]; Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling Gli1 with TA310536 at 1/250 dilution.

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  Immunocytochemistry/ Immunofluorescence - Anti-Gli1 antibody [EPR4523]; Immunofluorescent analysis of A673 cells labelling Gli1 with TA310536 at 1/250 dilution.

Specifications

Product Data

• Clone Name: EPR4523
• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:1000 - 1:10000; ICC/IF: 1:250 - 1:500; IHC-P: 1:250 - 1:500
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide corresponding to residues in human Gli-1 was used as an immunogen.
• Formulation: Preservative: 0.01% Sodium azide; Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
• Purification: Protein A purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: GLI family zinc finger 1
• Database Link:
  NP_001153517
  Entrez Gene 14632 MouseEntrez Gene 2735 Human
  UniProt ID P08151
• Background: Gli-1 is a member of the Kruppel family of zinc finger proteins. This transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop (1).
• Synonyms: GLI
• Note: Is unsuitable for Flow Cyt or IP.

Reference Data

• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway, Transcription Factors
• Protein Pathways: Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer