Plasma Kallikrein 1B (KLKB1) Rabbit Monoclonal Antibody [Clone ID: EPR5707]

CAT#: TA310647

Rabbit Monoclonal antibody against KLKB1

Product Images

Western blot - Anti-Plasma Kallikrein 1B antibody [EPR5707]; All lanes : Anti-Plasma Kallikrein 1B antibody [EPR5707] at 1/1000 dilution.Lane 1 : Human breast cancer lysate.Lane 2 : Human plasma lysate.Lane 3 : Human ovary lysate.Lane 4 : Fetal liver lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled Goat anti Rabbit IgG at 1/2000 dilution.Predicted band size : 71 kDa.Observed band size : 80 kDa.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasma Kallikrein 1B antibody [EPR5707]; TA310647, at 1/50 dilution, staining Plasma Kallikrein 1B in paraffin-embedded Human kidney tissue by Immunohistochemistry.

• 

  Other-Anti-Plasma Kallikrein 1B antibody [EPR5707] (TA310647); Equilibrium disassociation constant (KD).

Specifications

Product Data

• Clone Name: EPR5707
• Applications: Assay, IHC, WB
• Recommended Dilution: WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:50 - 1:100
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide corresponding to residues in the C-terminus of human KLKB1 was used as an immunogen.
• Formulation: pH: 7.40
  Preservative: 0.01% Sodium azide
  Constituents: 50% Glycerol, 0.05% BSA
• Purification: Tissue culture supernatant
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 71 kDa
• Gene Name: kallikrein B1
• Database Link:
  NP_000883
  Entrez Gene 16621 MouseEntrez Gene 25048 RatEntrez Gene 3818 Human
  UniProt ID P03952
• Background: KLKB1 (Plasma prekallikrein) is a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. It is synthesized in the liver and secreted into the blood as a single polypeptide chain. KLKB1 is converted to plasma kallikrein by factor XIIa by the cleavage of an internal Arg-Ile bond. This protein therefore is composed of a heavy chain and a light chain held together by a disulphide bond. The heavy chain originates from the amino-terminal end of the zymogen and contains 4 tandem repeats of 90 or 91 amino acids. Each repeat harbors a novel structure called the apple domain. The heavy chain is required for the surface-dependent pro-coagulant activity of KLKB1. The light chain contains the active site or catalytic domain of the enzyme and is homologous to the trypsin family of serine proteases. KLKB1 deficiency causes a prolonged activated partial thromboplastin time in patients (1).
• Synonyms: KLK3; PPK
• Note: Does not react with Mouse, Rat

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Complement and coagulation cascades