OGA Rabbit Monoclonal Antibody [Clone ID: EPR7154(B)]

CAT#: TA310690

Rabbit Monoclonal antibody against MGEA5

Product Images

Western blot - Anti-MGEA5 antibody [EPR7154 (B)]; All lanes : Anti-MGEA5 antibody [EPR7154 (B)] at 1/10000 dilution.Lane 1 : HeLa lysate.Lane 2 : BxPC-3 lysate.Lane 3 : JAR lysate.Lane 4 : Fetal brain lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 102 kDa.Observed band size : 130 kDa.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGEA5 antibody [EPR7154 (B)]; TA310690, at 1/100 dilution, staining MGEA5 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

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  Other-Anti-MGEA5 antibody [EPR7154 (B)] (TA310690); Equilibrium disassociation constant (KD).

Specifications

Product Data

• Clone Name: EPR7154(B)
• Applications: Assay, IHC, WB
• Recommended Dilution: WB: 1:10000 - 1:50000; IP: 1:100 - 1:500; IHC-P: 1:100 - 1:250
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide corresponding to residues in human MGEA5 was used as an immunogen.
• Formulation: pH: 7.20; Preservative: 0.01% Sodium azide; Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: meningioma expressed antigen 5 (hyaluronidase)
• Database Link:
  NP_001135906
  Entrez Gene 76055 MouseEntrez Gene 154968 RatEntrez Gene 10724 Human
  UniProt ID O60502
• Background: The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT, which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (1). MGEA5 also possesses hyaluronidase activity (2).
• Synonyms: MEA5; NCOAT; OGA
• Note: Is unsuitable for Flow Cyt or ICC.