Collagen II (COL2A1) Rabbit Polyclonal Antibody

CAT#: TA311649

Rabbit polyclonal Collagen II antibody

Product Images

Western blot analysis of extracts from COLO205 cells, using Collagen II antibody (#TA311649).The lane on the right is treated with the synthesized peptide.

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  Western blot analysis of extracts from 3T3 cells (Lane 2), using Collagen II Antibody. The lane on the left is treated with synthesized peptide.

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  Figure from citation: Western Blot of COL11 protein level by using anti-COL11 antibody in primary cultured mice chondrocytes. Dilution: 1:500 View Citation

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  Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Collagen II antibody (#TA311649).The picture on the right is treated with the synthesized peptide.

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  Immunofluorescence analysis of COS7 cells, using Collagen II antibody (#TA311649).The picture on the right is treated with the synthesized peptide.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:500~1:3000, IHC: 1:50~1:100, IF: 1:100~1:500, ELISA: 1:20000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized peptide derived from human Collagen II.
• Formulation: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: collagen type II alpha 1 chain
• Database Link:
  NP_001835
  Entrez Gene 12824 MouseEntrez Gene 25412 RatEntrez Gene 1280 Human
  UniProt ID P02458
• Synonyms: ANFH; AOM; COL11A3; SEDC; STL1
• Note: Collagen II antibody detects endogenous levels of total Collagen II protein.

Reference Data

• Protein Pathways: ECM-receptor interaction, Focal adhesion