NCF1 Rabbit Polyclonal Antibody

CAT#: TA311760

Rabbit polyclonal p47 phox/NCF1 (Ser345) antibody(Phospho-specific)

Product Images

Western blot analysis of extracts from HepG2 cells, treated with TNF (20ng/ml, 5mins), using p47 phox (Phospho-Ser345) antibody.The lane on the right is treated with the synthesized peptide.

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  Immunohistochemistry analysis of paraffin-embedded human brain tissue using p47 phox (Phospho-Ser345) antibody.The picture on the right is treated with the synthesized peptide.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:500~1:3000, IHC: 1:50~1:100, ELISA: 1:1000
• Reactivities: Human
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human p47 phox around the phosphorylation site of serine 345 (P-Q-SP-P-G).
• Formulation: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: neutrophil cytosolic factor 1
• Database Link:
  NP_000256
  Entrez Gene 653361 Human
  UniProt ID P14598
• Synonyms: NCF1A; NOXO2; p47phox; SH3PXD1A
• Note: p47 phox (Phospho-Ser345) antibody detects endogenous levels of p47 phox only when phosphorylated at serine 345.

Reference Data

• Protein Pathways: Chemokine signaling pathway, Fc gamma R-mediated phagocytosis, Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton