Chk1 (CHEK1) Rabbit Polyclonal Antibody

CAT#: TA311861

Rabbit polyclonal Chk1 (Ser296) antibody(Phospho-specific)

Product Images

Western blot analysis of extracts from HUVEC cells, treated with UV (15mins), using Chk1 (Phospho-Ser296) antibody.The lane on the right is treated with the synthesized peptide.

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  Immunohistochemistry analysis of paraffin-embedded human brain tissue using Chk1 (Phospho-Ser296) antibody.The picture on the right is treated with the synthesized peptide.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:500~1:3000, IHC: 1:50~1:100, ELISA: 1:5000
• Reactivities: Human
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Chk1 around the phosphorylation site of serine 296 (I-Q-SP-N-L).
• Formulation: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: checkpoint kinase 1
• Database Link:
  NP_001265
  Entrez Gene 1111 Human
  UniProt ID O14757
• Synonyms: CHK1
• Note: Chk1 (Phospho-Ser296) antibody detects endogenous levels of Chk1 only when phosphorylated at serine 296.

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency
• Protein Pathways: Cell cycle, p53 signaling pathway