IKK alpha (CHUK) Rabbit Polyclonal Antibody

CAT#: TA311872

Rabbit polyclonal IKK-alpha (Ser176)/IKK-beta (Phospho-Ser177) antibody(Phospho-specific)

Product Images

Western blot analysis of extracts from 3T3 cells, treated with TNF (20ng/ml, 30mins), using IKK-a (Phospho-Ser176)/IKK-Ã? (Phospho-Ser177) antibody.The lane on the right is treated with the synthesized peptide.

• 

  Western blot analysis of extracts from A549 cells (Lane 2) and 3T3 cells (Lane 3), using IKK-a (Phospho-Ser176) /IKK-Ã? (Phospho-Ser177) Antibody. The lane on the left is treated with synthesized peptide.

• 

  Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using IKK-a (Phospho-Ser176)/IKK-β (Phospho-Ser177) antibody.The picture on the right is treated with the synthesized peptide.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:500~1:3000, IHC: 1:50~1:100, ELISA: 1:1000
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human IKK-a around the phosphorylation site of serine 176/177 (Q-G-SP-L-C).
• Formulation: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: conserved helix-loop-helix ubiquitous kinase
• Database Link:
  NP_001269
  Entrez Gene 12675 MouseEntrez Gene 309361 RatEntrez Gene 1147 Human
  UniProt ID O15111
• Synonyms: IKBKA; IKK-alpha; IKK1; IKKA; NFKBIKA; TCF16
• Note: IKK-. (Phospho-Ser176)/IKK-. (Phospho-Ser177) antibody detects endogenous levels of IKK-. only when phosphorylated at serine 176/177.

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Cytosolic DNA-sensing pathway, Epithelial cell signaling in Helicobacter pylori infection, MAPK signaling pathway, NOD-like receptor signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway