EIF4E Rabbit Polyclonal Antibody

CAT#: TA311935

Rabbit polyclonal elF4E (Ser209) antibody(Phospho-specific)

Product Images

Western blot analysis of extracts from 293 cells, using elF4E (Phospho- Ser209) antibody.The lane on the right is treated with the synthesized peptide.

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  Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using elF4E (phospho- Ser209) antibody.The picture on the right is treated with the synthesized peptide.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:500~1:3000, IHC: 1:50~1:100, ELISA: 1:20000
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human elF4E around the phosphorylation site of serine 209 (S-G-SP-T-T).
• Formulation: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: eukaryotic translation initiation factor 4E
• Database Link:
  NP_001959
  Entrez Gene 13684 MouseEntrez Gene 117045 RatEntrez Gene 1977 Human
  UniProt ID P06730
• Synonyms: AUTS19; CBP; eIF-4E; EIF4E1; EIF4EL1; EIF4F
• Note: elF4E (phospho-Ser209) antibody detects endogenous levels of elF4E only when phosphorylated at serine 209.

Reference Data

• Protein Pathways: Insulin signaling pathway, mTOR signaling pathway