PKC zeta (PRKCZ) Rabbit Polyclonal Antibody

CAT#: TA312044

Rabbit polyclonal PKC zeta (Thr410) antibody(Phospho-specific)

Product Images

Western blot analysis of extracts from NIH/3T3 cells treated with PMA (125ng/ml, 30mins), using PKC? (phospho-Thr410) antibody.The lane on the right is treated with the synthesized peptide.

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  Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using PKC? (phospho-Thr410) antibody.The picture on the right is treated with the synthesized peptide.

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  Immunofluorescence analysis of HeLa cells, using PKC? (phospho-Thr410) antibody.The picture on the right is treated with the synthesized peptide.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:500~3000, IHC: 1:50~100, IF: 1:100~1:500, ELISA: 1:20000
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human PKC? around the phosphorylation site of threonine 410 (T-S-TP-F-C).
• Formulation: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: protein kinase C zeta
• Database Link:
  NP_002735
  Entrez Gene 18762 MouseEntrez Gene 25522 RatEntrez Gene 5590 Human
  UniProt ID Q05513
• Synonyms: PKC-ZETA; PKC2
• Note: PKC. (phospho-Thr410) antibody detects endogenous levels of PKC. only when phosphorylated at threonine 410.

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway, Endocytosis, Insulin signaling pathway, Tight junction, Type II diabetes mellitus