SMAD3 Rabbit Polyclonal Antibody

CAT#: TA312265

Rabbit polyclonal Smad3 (Ab-204) antibody

Product Images

Western blot analysis of extracts from HUVEC cells, using Smad3 (Ab-204) antibody.The lane on the right is treated with the synthesized peptide.

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  Immunohistochemistry analysis of paraffin-embedded human colon colon tissue using Smad3 (Ab-204) antibody.The picture on the right is treated with the synthesized peptide.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:500~1:3000, IHC: 1:50~1:100, ELISA: 1:5000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human Smad3 around the phosphorylation site of serine 204 (A-G-SP-P-N).
• Formulation: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: SMAD family member 3
• Database Link:
  NP_005893
  Entrez Gene 17127 MouseEntrez Gene 25631 RatEntrez Gene 4088 Human
  UniProt ID P84022
• Synonyms: HSPC193; HsT17436; JV15-2; LDS1C; LDS3; MADH3
• Note: Smad3 (Ab-204) antibody detects endogenous levels of total Smad3 protein.

Reference Data

• Protein Families: Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transcription Factors
• Protein Pathways: Adherens junction, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Pancreatic cancer, Pathways in cancer, TGF-beta signaling pathway, Wnt signaling pathway