NF-kB p65 (RELA) Rabbit Polyclonal Antibody

CAT#: TA312411

Rabbit polyclonal NF-kB p65 (Ser529) antibody(Phospho-specific)

Product Images

Western blot analysis of extracts from JurKat cells and HeLa cells, using NF-κB p65 (phospho-Ser529) antibody.The lane on the right is treated with the synthesized peptide.

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  Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NF-κB p65 (phospho-Ser529) antibody.The picture on the right is treated with the synthesized peptide.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:500~1:3000, IHC: 1:50~1:100, ELISA: 1:10000
• Reactivities: Human, Mouse
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human NF-?B p65 around the phosphorylation site of serine 529 (L-L-SP-G-D).
• Formulation: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: RELA proto-oncogene, NF-kB subunit
• Database Link:
  NP_068810
  Entrez Gene 19697 MouseEntrez Gene 5970 Human
  UniProt ID Q04206
• Synonyms: NFKB3; p65
• Note: NF-.B p65 (Ab-529) antibody detects endogenous levels of NF-.B p65 protein only when phosphorylated at serine 529.

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Cytosolic DNA-sensing pathway, Epithelial cell signaling in Helicobacter pylori infection, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway