EIF4B Rabbit Polyclonal Antibody

CAT#: TA313174

Rabbit polyclonal eIF4B (Ser422) antibody(Phospho-specific)

Product Images

Western blot analysis of extracts from NIH/3T3cells, using eIF4B (Phospho-Ser422) antibody.The lane on the right is treated with the synthesized peptide.

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  Western blot analysis of extracts from 3T3 cells (Lane 2), using eIF4B (Phospho-Ser422) Antibody. The lane on the left is treated with synthesized peptide.

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  Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using eIF4B (Phospho-Ser422) antibody.The picture on the right is treated with the synthesized peptide.

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  Immunofluorescence analysis of NIH/3T3 cells, using eIF4B (Phospho-Ser422) antibody.The picture on the right is treated with the synthesized peptide.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:500~1:3000, IHC: 1:50~1:100, IF: 1:100~1:500, ELISA: 1:40000
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human eIF4B around the phosphorylation site of serine 422 (T-G-SP-E-S).
• Formulation: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: eukaryotic translation initiation factor 4B
• Database Link:
  NP_060977
  Entrez Gene 55378 Entrez Gene 1975 Human
  UniProt ID P23588
• Synonyms: EIF-4B; PRO1843
• Note: eIF4B (Phospho-Ser422) antibody detects endogenous levels of eIF4B only when phosphorylated at serine 422.