AKT1 Rabbit Polyclonal Antibody

CAT#: TA313265

Rabbit polyclonal Akt(Thr308) antibody(Phospho-specific)

Product Images

Western blot analysis using Akt (Phospho-Thr308) Antibody: Line1: The extracts from 293 cells untreated; Line2: The extracts from 293 cells treated with insulin.The lane on the right is treated with the synthesized peptide.

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  Immunohistochemical analysis of paraffin-embedded lung carcinoma, using Akt (Phospho-Thr308) Antibody. Left: Untreated; Right: Treated with synthesized phosphopeptide.The picture on the right is treated with the synthesized peptide.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:500~1:3000, IHC: 1:50~1:100, ELISA: 1:10000
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Akt around the phosphorylation site of Threonine 308 (M-K-TP-F-C).
• Formulation: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: AKT serine/threonine kinase 1
• Database Link:
  NP_005154
  Entrez Gene 11651 MouseEntrez Gene 24185 RatEntrez Gene 207 Human
  UniProt ID P31749
• Synonyms: AKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA
• Note: Akt(Phospho-Thr308) Antibody detects endogenous levels of Akt only when phosphorylated at Threonine 308.

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Glioma, Insulin signaling pathway, Jak-STAT signaling pathway, MAPK signaling pathway, Melanoma, mTOR signaling pathway, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Renal cell carcinoma, Small cell lung cancer, T cell receptor signaling pathway, Tight junction, Toll-like receptor signaling pathway, VEGF signaling pathway