AKT1 Rabbit Polyclonal Antibody

CAT#: TA313266

Rabbit polyclonal Akt (Ser473) antibody(Phospho-specific)

Product Images

Western blot analysis of extracts from K562, using Akt (Phospho-Ser473) Antibody.The lane on the right is treated with the synthesized peptide.

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  Western blot analysis of extracts from JK cells (Lane 2), using Akt (Phospho-Ser473) Antibody. The lane on the left is treated with synthesized peptide.

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  Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Akt (Phospho-Ser473) Antibody.The picture on the right is treated with the synthesized peptide.

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  Immunofluorescence analysis of A549 cells, using Akt (Phospho-Ser473) AntibodyThe picture on the right is treated with the synthesized peptide.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:500~1:3000, ELISA: 1:1000, IHC: 1:50~1:200
• Reactivities: Human
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Akt
• Formulation: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: AKT serine/threonine kinase 1
• Database Link:
  NP_005154
  Entrez Gene 207 Human
  UniProt ID P31749
• Synonyms: AKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA
• Note: Akt (Phospho- Ser473) Antibody detects endogenous levels of Akt only when phosphorylated at Ser473.

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Glioma, Insulin signaling pathway, Jak-STAT signaling pathway, MAPK signaling pathway, Melanoma, mTOR signaling pathway, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Renal cell carcinoma, Small cell lung cancer, T cell receptor signaling pathway, Tight junction, Toll-like receptor signaling pathway, VEGF signaling pathway