RUNX1 Rabbit Polyclonal Antibody

CAT#: TA313289

Rabbit polyclonal AML1 / RUNX1 (Ser435) antibody(Phospho-specific)

Product Images

Western blot analysis of extracts from HepG2 cells, treated with PMA (125ng/ml, 30mins), using AML1 (Phospho-Ser435) antibody.The lane on the right is treated with the synthesized peptide.

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  Western blot analysis of extracts from 293 cells (Lane 2), HeLa cells (Lane 3) and HepG2 cells (Lane 4), using AML1 (Phospho-Ser435) Antibody. The lane on the left is treated with synthesized peptide.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500~1:3000, ELISA: 1:10000
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human AML1 around the phosphorylation site of serine 435 (S-N-SP-P-T).
• Formulation: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: runt related transcription factor 1
• Database Link:
  NP_001745
  Entrez Gene 12394 MouseEntrez Gene 50662 RatEntrez Gene 861 Human
  UniProt ID Q01196
• Synonyms: AML1; AML1-EVI-1; AMLCR1; CBFA2; EVI-1; PEBP2aB
• Note: AML1 (Phospho-Ser435) antibody detects endogenous levels of AML1 only when phosphorylated at serine 435.

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Chronic myeloid leukemia, Pathways in cancer