14-3-3 zeta (YWHAZ) Rabbit Polyclonal Antibody

CAT#: TA314679

Rabbit polyclonal 14-3-3 zeta (Ser58) antibody(Phospho-specific)

Product Images

Western blot analysis of extracts from NIH/3T3 cells treated with UV (30mins), using 14-3-3 zeta (phospho-Ser58) antibody.The lane on the right is treated with the synthesized peptide.

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  Western blot analysis of extracts from A549 cells (Lane 2) and 3T3 cells (Lane 3), using 14-3-3 zeta (Phospho-Ser58) Antibody. The lane on the left is treated with synthesized peptide.

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  Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 14-3-3 ? (phospho-Ser58) antibody.The picture on the right is treated with the synthesized peptide.

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  Immunofluorescence analysis of HeLa cells, treated with PMA (125ng/ml, 30mins), using 14-3-3 ? (phospho-Ser58) antibody.The picture on the right is treated with the synthesized peptide.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:500~1000, IHC: 1:50~100, IF: 1:100~1:500, ELISA: 1:40000
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human 14-3-3 ? around the phosphorylation site of serine 58 (R-S-SP-W-R)
• Formulation: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta
• Database Link:
  NP_663723
  Entrez Gene 22631 MouseEntrez Gene 25578 RatEntrez Gene 7534 Human
  UniProt ID P63104
• Synonyms: 14-3-3-zeta; HEL-S-3; HEL4; KCIP-1; YWHAD
• Note: 14-3-3 . (phospho-Ser58) antibody detects endogenous levels of 14-3-3 . only when phosphorylated at serine 58.

Reference Data

• Protein Pathways: Cell cycle, Neurotrophin signaling pathway, Oocyte meiosis, Pathogenic Escherichia coli infection