H3FA (HIST1H3A) Rabbit Polyclonal Antibody

CAT#: TA316501

Rabbit polyclonal anti-Histone H3.1 (Ser10) antibody (Phospho-specific)

Product Images

Western blot - Histone H3 (phospho S10) antibody. All lanes : Anti-Histone H3 (phospho S10) antibody. Lane 1 : EGF + Calyculin treated HeLa cells + phospho-peptide. Lane 2 : EGF + Calyculin treated HeLa cells. No phosphopeptide. Lysates/proteins at 30 ug per lane. Predicted band size : 15 kDa

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  Immunohistochemistry (Paraffin-embedded sections) - Histone H3 (phospho S10) antibody. staining human breast carcinoma by IHC-P (left hand panel). The right hand panel shows staining in the presence of immunizing phosphopeptide.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:500~1:3000, IHC: 1:50~1:100, ELISA: 1:10000
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Histone H3.1 around the phosphorylation site of serine 10 (R-K-SP-T-G).
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: histone cluster 1, H3a
• Database Link:
  NP_003520
  Entrez Gene 8350 Human
  UniProt ID P68431
• Synonyms: A; H3; H3FA
• Note: Histone H3.1 (phospho-Ser10) antibody detects endogenous levels of Histone H3.1 only when phosphorylated at serine 10.

Reference Data

• Protein Pathways: Systemic lupus erythematosus