PARP1 Mouse Monoclonal Antibody [Clone ID: 7A10]

CAT#: TA318859

Mouse monoclonal anti-PARP antibody, clone 7A10

Product Images

Western blot analysis using PARP mouse mAb against Jurkat (1), K562 (2), HeLa (3), Raji (4), THP-1 (5) and SW620 (6) cell lysate.

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  Flow cytometric analysis of Jurkat cells using anti-PARP mAb (green) and negative control (purple).

Specifications

Product Data

• Clone Name: 7A10
• Applications: FC, WB
• Recommended Dilution: WB: 1:500-2000, IF: 1:200-400
• Reactivities: Human
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: A synthetic peptide corresponding to residues before the cleavage site of human PARP-1 was used as immunogen. The antibody only recognizes full-length form (p116) of PARP-1.
• Formulation: Supplied in 10 mM HEPES sodium (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol, and 0.03% sodium azide
• Purification: N/A
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: poly(ADP-ribose) polymerase 1
• Database Link:
  NP_001609
  Entrez Gene 142 Human
  UniProt ID P09874
• Background: PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress. This protein can be cleaved by many ICE-like Caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis.
• Synonyms: ADPRT; ADPRT 1; ADPRT1; ARTD1; pADPRT-1; PARP; PARP-1; PPOL

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Base excision repair