MCP1 (CCL2) Rabbit Polyclonal Antibody

CAT#: TA319048

Rabbit polyclonal anti-CCL2 (MCP-1) antibody

Product Images

Western blot analysis of MCP-1 in LPS treated RAW cell lysate.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 0.5-2 ug/ml
• Reactivities: Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: E. coli-expressed rat MCP-1 (a.a. 1-73)
• Formulation: 100 µg (0.5 mg/ml) immunoaffinity purified rabbit anti-rat MCP-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% sodium azide.
• Concentration: lot specific
• Purification: Affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: C-C motif chemokine ligand 2
• Database Link:
  NP_002973
  Entrez Gene 20296 MouseEntrez Gene 24770 Rat
  UniProt ID P13500
• Background: Rat MCP-1/JE (monocyte chemoattractant protein-1) is a 148-amino acid CC chemokine with a N-terminal sequence of 29 residues as a signal sequence. It was originally cloned from Con-A-stimulated rat spleen cDNA library. This rat MCP-1/JE is 49-amino acid longer than human MCP-1 at 3’-end. The 3’-end is a serine and threonine rich zone, which is probably responsible for the extensive O-glycosylation and which explains for the higher molecular weight (25 kDa). In vitro, MCP-1/JE is chemotactic for monocytes as well as lymphocytes and basophils, but not for neutrophiles. MCP-1/JE is produced by a wide range of cell types as a reaction to diverse inflammatory stimuli.
• Synonyms: GDCF-2; HC11; HSMCR30; MCAF; MCP-1; MCP1; SCYA2; SMC-CF

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Chemokine signaling pathway, Cytokine-cytokine receptor interaction, NOD-like receptor signaling pathway