Jagged1 (JAG1) Rabbit Polyclonal Antibody

CAT#: TA319134

Rabbit polyclonal anti-Jagged 1 antibody

Product Images

WB using anti-Jagged-1 antibody shows detection of Jagged-1 protein in various whole cell lysates: human brain (lane 1), human kidney (lane 2), human liver (lane 3), and mouse liver (lane 5). Lane 4 contained sample buffer only.Each lane contains approximately 20 ug of lysate. Primary antibody was used at a 1:500 dilution. Predicted molecular weight is 134 kDa.

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  Immunohistochemical staining of human cervical cancer tissue (40X magnification) using Protein A purified anti-Jagged-1 antibody. Tissue was fixed with formalin and embedded in paraffin. Hematoxylin was used to counter-stain cells. A 1:100 dilution of primary antibody was used. Personal Communication. Martin Kast Laboratory.

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  Immunofluorescence microscopy using Protein A purified anti-Jagged-1 antibody of human corneal epithelial cells. Primary antibody was used at a 1:500 dilution. The Jagged1 (green staining) is local-ized to the cytoplasm and is consistent with reports in the literature. The nucleus is stained with Bis benzimide (blue). Personal Communication. Aihua Ma, Univdersity of Cardiff.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ELISA: 1:20,000 - 1:100,000, WB: 1:500 - 1:2,000, IHC: 1:100 - 1:500, IF: 1:200 - 1:1,000
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 110-125 of human Jagged-1protein.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: jagged 1
• Database Link:
  NP_000205
  Entrez Gene 16449 MouseEntrez Gene 182 Human
  UniProt ID P78504
• Synonyms: AGS; AHD; AWS; CD339; HJ1; JAGL1
• Note: Anti Jagged 1 Antibody recognizes the jagged 1 protein encoded by the JAG1 gene, that is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signaling through notch 1 has also been shown to play a role in hematopoies.

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Notch signaling pathway