Cyclin A2 (CCNA2) Rabbit Polyclonal Antibody

CAT#: TA319146

Rabbit polyclonal anti-Cyclin A antibody

Product Images

Western Blot of Rabbit anti-Cyclin A antibody. Lane 1: T98G cells treated with normal (n). Lane 2: T98G cells treated with scrambled (scr). Lane 3: T98G cells treated with PIN1 knockdown (kd1). Load: 25 ug per lane. Primary antibody: Cyclin A antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 60kDa for Cyclin A.

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  Immunohistochemistry of Rabbit Anti-Cyclin A antibody. Tissue: human testes tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Cyclin A antibody at 1:500 for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Cyclin A is nuclear and cytoplasmic. Staining: Cyclin A as precipitated red signal with hematoxylin purple nuclear counterstain.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1:5,000 - 1:20,000, WB: 1:500 - 1:5,000, IP: 1:100
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Anti-Cyclin-A Antibody was produced by repeated immunizations with a recombinant protein corresponding to the human cyclin A.
• Formulation: None
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: cyclin A2
• Database Link:
  NP_001228
  Entrez Gene 12428 MouseEntrez Gene 114494 RatEntrez Gene 890 Human
  UniProt ID P20248
• Synonyms: CCN1; CCNA
• Note: Anti-Cyclin A detects cyclin a. Cyclin A is a member of the cyclin family. Cyclin A binds to S phase Cdk2 and is required for the cell to progress through the S phase. Cyclin A/ Cdk2 is inhibited by the complex p21CIP. Anti-cyclin-a Antibody is ideal for investigators involved in Cell Signaling, cell biology and Signal Transduction research.

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Cell cycle, Progesterone-mediated oocyte maturation