PCNA Rabbit Polyclonal Antibody

CAT#: TA319163

Rabbit polyclonal anti-PCNA antibody

Product Images

WB using Anti-Alga PCNA antibody shows detection of a predominant band at ~29 kDa corresponding to PCNA (arrowhead lane 2). Peptide competition effectively eliminates specific band reactivity (lane 1). For peptide competition, the antibody was pre-incubated with 100 ug (excess) of the peptide for 2h at 37°C followed by overnight incubation at 4°C with 100 ug (excess) of the peptid. Primary antibody diluted to 1:2001000.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:10,000 - 1:50,000, WB: 1:5,000 - 1:32,000, IP: 1:100
• Reactivities: Algal
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 55-71 of Isochrysis galbana PCNA.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: proliferating cell nuclear antigen
• Database Link:
  NP_002583
  Entrez Gene 5111 Human
  UniProt ID P12004
• Synonyms: ATLD2
• Note: PCNA, or Proliferating Cell Nuclear Antigen, is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA is a nuclear protein.

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Base excision repair, Cell cycle, DNA replication, Mismatch repair, Nucleotide excision repair